IDENTIFIKASI BAKTERI DARI LIMBAH CAIR MINYAK BUMI PADA SEKTOR GS CHEVRON KECAMATAN RIMBA MELINTANG ROKAN HILIR SEBAGAI MODUL PEMBELAJARAN KONSEP PROKARIOT PADA MATA KULIAH MIKROBIOLOGI DASAR
Sari
ABSTRACT
This research was aimed at studying the identification of bacteria from the liquid waste of petroleum from August to October 2014. The result of research was used to development a learning module in basic microbiology course. This research was implemented in 2 steps, they are Laboratory research and development a learning module by use of ADDIE (Analysis, Design, Development, Implementation and Evaluation) development. Isolation of bacteria was under taken using thinning methods (platting methods) grown at solid media Stone Mineral Salt Solution Extract Yeast (SMSSe) added with crude oil as selective media. Colonies observed on the media after incubation on the temperature 28-300C for 24-48 hours were identified based on macroscopic observations, microscopic and biochemical tests to ensure that the bacteria identified was absolutely petroleum bacteria. The research result carried out 3 genera of bacteria namely Enterobacter, Pseudomonas and Klebsiella. The result of research is developed as learning module in the concept of prokaryot to enrich teaching materials in basic microbiology course.
Key words: Identification, SMSSe Media, Learning Module
Teks Lengkap:
PDFReferensi
Bambang Yudono, Sri Pertiwi Estuningsih, M. Said, Sabarudin dan Adipati Napoleon. 2013. Eksplorasi Bakteri Indigen Pendegradasi Limbah Minyak Bumi di Wilayah PT Pertamina UBEP Limau Muara Enim. Prosiding Semirata FMIPA Universitas Lampung. Lampung.
Didi Saidi, Anas Iswand, Noegroho Hadi dan Dwi Andreas Santoso. 1999. Kemampuan Bakteri dari Ekosistem Air Hitam Kalimantan Tengah dalam Merombak Minyak Bumi dan Solar. Jurnal Ilmu Tanah dan Lingkungan. 2(2):1-7. FAPERTA Universitas Pembangunan Nasional Veteran. Yogyakarta.
Eko Putro Widoyoko. 2012. Teknik Penyusunan Instrumen Penelitian. Pustaka Pelajar. Yogyakarta.
Feliatra. 2001. Buku Ajar Mikrobiologi Laut. Pusat Penelitian Kawasan Pantai dan Perairan Universitas Riau. Pekanbaru.
Irda Sayuti dan Sri Wulandari. 2012. Silabus Mata Kuliah Mikrobiologi Dasar. FKIP Biologi Universitas Riau. Pekanbaru.
Karwati. 2009. Degradasi Hidrokarbon pada Tanah tercemari Minyak Bumi dengan Isolat A10 dan D8.Departemen Kimia. FMIPA. Institut Pertanian Bogor. Bogor.
Nur Hidayat, Masdiana C. Padaga dan Sri Suhartini. 2006. Mikrobiologi Industri. ANDI. Yogyakarta.
M. J. Pelczar and E.C.S. Chan. 1988. Dasar-Dasar Mikrobiologi. UI Press. Jakarta. Peraturan Menteri Lingkungan Hidup Nomor 19 Tahun 2010. Tentang Baku Mutu Air Limbah bagi Usaha dan/atau Kegiatan Minyak dan Gas serta Panas Bumi. MENLH. Jakarta.
Riau Pos.com. 2013. Potensi dan Tantangan Nasionalisasi Migas di Tengah Penurunan Produksi di Riau. Menakar Kemampuan Perusahaan Nasional-atau Daerah (online).http://riaupos.com/berita.menakar-kemampuan-perusahaan-nasional-atau-daerah.html. (diakses 10 April 2014).
Robert S. Breed, E.G.D. Murray and Nathan R. Smith. 1957. Bergey’s Manual of Determinative Bacteriology seventh edition. The Williams and Wilkins Company. United State of America.
W. Dick and L. Carey. 2005. The Systematic Design of Instruction. Perason. Boston.
Yustina. 2010. Modul Pembelajaran. FKIP Universitas Riau. Pekanbaru.
Refbacks
- Saat ini tidak ada refbacks.