Pengaruh Pemberian Taurin terhadap Gambaran Histopatologi Paru Mencit (Mus musculus) yang Diinduksi Karsinogen Benzo(α)Piren secara In Vivo

Arini Pradita Roselyn, Endang Linirin Widiastuti, G. Nugroho Susanto, Sutyarso '

Abstract


Lung cancer is a disease that causes high mortality. Drugs used to prevent and cure cancer mostly causes intoxicity to the normal tissues due to its less effectiveness. Therefore, it is necessary to find out any agent or substance which works much more effective and safe for cancer treatment. The aim of the study was to elucidate the role of taurine on the lung tissue of mice (Mus musculus) induced by carcinogenic benzo(α)pyrene. The experiment was conducted in a completely randomized design with 5 replications. Six treatment groups were perfomed. Group I was given 0.2 mL of corn oil and given aquadest until the end of the study period, group II was induced by benzo(α)pyrene without administration of taurine, group III before induced with benzo(α)pyrene, was given taurine dosage 7.8 mg/BW/day for two weeks, group IV after induced benzo(α)pyrene, was given taurine with dosage 3.9 mg/BW/day, group V after induced benzo(α)pyrene, was given taurine with dosage7. 8 mg/BW/day, group VI after induced with benzo(α)pyrene, was given taurine with dosage 15.6 mg/BW/day. The results of the Kruskal-Wallis analysis and one way ANOVA with LSD (p>0,05) showed that taurine reduced lung tissue damage 72.73% due to the administration of benzo(α)pyrene of 0.3 mg/BW/day. In addition, the effective dose of taurine reduce lung tissue damage was 15.6 mg/BW/day.


Keywords


benzo(α)phyren, histhopatology, lung, taurine

Full Text:

PDF

References


Abdih, H., Kelly C.J., Bouchier-Hayes, D., Mary Barry & Kearns, S. 2000. Taurine Prevents Interleukin-2- Induced Acute Lung Injury in Rats. European Surgical Research 32(6): 347–352.

Adam, R.D & Victor, M. 2000. The Syndrome of Aseptic Meningitis, Non Viral Infection of The Nervous System. In : Principles of Neurology. Mc Graw Hill.Toronto 589–629.

Akrom. 2012. Mekanisme Kemopreventif Ekstrak Heksan Biji Jinten Hitam (Nigella sativa Lor Pada Tikus Sprague Dawley Diinduksi 7,12 Dimethylbenz(α)antracene: Kajian Antioksidan dan Immunomodulator. Disertasi. Yogyakarta: Universitas Gadjah Mada.

Ali, H.T. 2007. Beneficial Efects Of Nigella sativa On The Testis Tissues Of Mice Exposed to UV Irradiation. Biology Departement/ Educatioan College/ Mosul University.

Droge, W. 2002. Free radicals in the physiological control of cell function. Physiol Rev 82(1): 47–95.

Gordon, R.E., A.A. Shaked, D.F & Solano. 1986. Taurine protects hamster bronchioles from acute NO2-induced alterations. A histologic, ultrastuctural and freeze-fracture study. Am J Pathol 125(3):585–600.

Greaves, P. 2000, Histopathology of Preclinical Toxicity Studies Interpretation and Relevance in Drug Safety Evaluation, Second Edition, 372–380, Amsterdam: Elsevier.

Gurujeyalakshmi, G., Wang, Y & Giri, S.N. 2000. Taurine and niacin block lung injury and fibrosis by down- regulating bleomycin-induced activation of transcription nuclear factor-kappaB in mice. J Pharmacol Exp Ther 293(1): 82–90.

Gustanti, E. 1999. Uji Efek Anti Kanker Dadih S. lactis Terhadap Mencit yang Diinduksi dengan Benzopiren. Skripsi Sarjana. Fakultas Matematika dan Ilmu Pengetahuan Alam. Padang: Universitas Andalas.

Guyton & Hall. 1996. Fisiologi Manusia dan Mekanisme Penyakit, Edisi 9. Jakarta: Penerbit Buku Kedokteran EGC.

Guz, G., E. Oz, N & Lortlar. 2007. The effect of taurine on renal ischemia-reperfusion injury. Amino Acids 32(3): 405–411.

Hansel, T.T & Barnes, P.J. 2004. An Atlas of Chronic Obstructive Pulmonary Disease. London: Parthenon Publishing Group. pp: 22–36.

Harbinson, R.D. 2001. The Basic Science of Poison in Cassaret and Doulls Toxicology. New York: Mac Millan Publishing.

Harrigan, J.A., McGarrigle, B.P., Sutter, T.R & Olson,

J.R. 2006. Tissue Spesific Induction Of Cytochrome P450 (CYP) 1A1 1B1 in Rat Liver and Lung Following In Vitro (tissue slice) an In Vivo Exposure to benzo(α)pyrene. Toxicol In Vitro 20(4): 426–438.

Harun, S & Sally, N. 2009. Edem Paru Akut. In: Sudoyo, AW, Setiyohadi B, Alwi I, Simadibrata M, SetiatiS,editor. BukuAjarIlmuPenyakitDalam 5th ed. Jakarta: Pusat Penerbitan Departemen Ilmu Penyakit Dalam Fakultas Kedokteran Universitas Indonesia. p. 1651–3.

Macnee, W. 2005. Pulmonary and Systemic Oxidant/ Antioxidant Imbalance in Chronic Obstructive Pulmonary Disease. http://www.atsjournals.org. (13 Maret 2015).

Male, D.J. Prostoff, D. Broth & Ivan Roitt. 2006. Immunology 7ed. Canada: Mosby Inc.

Mansyur. 2002. Toxicology. Selective Toxicity and Test. Medan : Universitas Sumatera Utara digital library.

Marianti, A. 2009. Aktivitas Antioksidan Jus Tomat pada Pencegahan Kerusakan Jaringan Paru-Paru Mencit yang Dipapar Asap Rokok. Biosaintifika 1(1): 1–10.

Nugraha, L.S.A. 2011.Cara dan Rute Pemberian Obat Pada Hewan Percobaan Mencit. Semarang: Akademi Farmasi Theresiana.

Nurliani, A., Santoso, H.B & Rusmiati. 2012. Efek Antioksidan Ekstrak Bulbus Bawang Dayak (Eleutherine palmifolia ) pada Gambaran Histopatologis Paru-paru Tikus yang Dipapar Asap Rokok. Bioscintiae 9(1): 60–69.

Perhimpunan Dokter Paru Indonesia (PDPI). 2003. Kanker Paru, Pedoman Diagnosis dan Penatalaksanaan di Indonesia. Jakarta: Balai Penerbit FKUI.

Quinn, A., Wong, C., Younus, J., Dranitsaris, G., Goel, R & Trudeau, M. 2009. Canadian Pattern of Care for Anemia: Comparison of Chemotherapies in Adjuvant Breast Cancer Setting. American Association for Cancer Research.

Renne, R.A., Dungworth D.L., Keenan C.M., Morgan K.T., Hahn F.F & Schwartz L.W. 2003. Non proliferative lesions of the respiratory tract in rats [serial online]. Available from: URL: http://www.toxpath.org/ssdnc/ RespiratoryNonprolifRat.pdf. (3 Maret 2015).

Robbins. 2007. Buku Ajar Patologi. Volume 2. Edisi 7. Jakarta: Penerbit buku Kedokteran EGC.

Rubin, E. 2009. Essential Pathology, 3rd ed. USA: Lippicott Williams & Wilkins.

Schuller-Lewis, G & Qinn, M.R. 1994. Taurine protects against oxidant-induced lung injury: Possible mechanism(s) of action. Adv Exp Med Biol 359: 31– 39.

Shao, A & Hathcock, J.N. 2008. Risk assessment for the amino acids taurine, l-glutamine and l-arginine. Regul Toxicol Pharmacol 50(3): 376–399.

Sugitha, I.M & Djalil, M. 1989. Susu, Penanganan dan Teknologinya. Fakultas Peternakan. Padang: Universitas Andalas.

Venkatesan, N & Chandrakasan, G. 1994. In-vivo administration of taurine and niacin modulate cyclophosphamide induced lung injury. Eur J Pharmacol 292(1): 75–80.

Walaszek, Z., Hanausek, M., Zoltaszek, R & Siaga, T.J. 2004. Inhibitory effect of post-initiation dietery D- Glucarate on benzo[α]pyrene induced inflammation during lung tumorigenesis in A/J mice. Proc. Amer Assoc Cancer Res 45: 132–141.

Wang, Q., Wang, Y., Hyde, D.M., Gotwals, P.J., Koteliansky, V.E., Ryan, S.T & Giri, S.N. 1999. Reduction of Bleomycin Induced Lung Fibrosis by Transforming Growth Factor β Sluble Receptor in Hamster. Thorax 54: 805–812.

Yao, H., Yang, S.R., Kode, A., Rajendrasozhan, S., Caito, S., Adenuga, D., Henry, R., Edirisinghe, I & Rahman, I. 2007. Redox regulation of lung inflammation: Role of NADPH oxidase and NF-kB Signalling. Bochem Soc Trans 35(5): 1151-1155.




DOI: http://dx.doi.org/10.31258/jni.17.1.22-32

Refbacks

  • There are currently no refbacks.


Copyright (c) 2017 Arini Pradita Roselyn, Endang Linirin Widiastuti, G. Nugroho Susanto, Sutyarso '

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.