Kestabilan Inokulan Azotobacter selama Penyimpanan pada Dua Suhu

Reginawanti Hindersah, Rija Sudirja

Abstract


Azotobacter might be used as biological agents in bioremediation of heavy metal-contaminated soil since this rhizobacteria produceexopolysachharides (EPS) that mobilize soil heavy metals, and phytohormones that regulate root growth. So that heavy metal uptake bythe roots could be increased. The objective of this research was to verify the stability of EPS and phytohormones in Azotobacter liquidinoculants during four months in different temperature storage. Liquid inoculants has been produced in EPS-induced media and stored in200C and room temperature (24-270C) during four months. The results showed that the better temperature storage was room temperatureinstead of 20 0C since pH, total N, and EPS and phytohormones content was relatively stable during storage.


Keywords


azotobacter, exopolysaccharide, inoculant quality, phytohormones, temperature storage

Full Text:

PDF

References


Abbass, Z. & Okon, Y. 1993. Plant Growth Promotion by Azotobacter paspali in The Rhizosphere. Soil Biol. Biochem 8: 1075-1083.

Alloway, B.J. 1995. Cadmium. Di dalam: Alloway, B.J (ed). Heavy Metals in Soils. Blackie Academic & Professional. Glasgow.

Coenen, C. & Lomax, T.L. 1998. The Diageotropica Gene Differentially Affect Auxin and Cytokinin Responses Throughout Development in Tomato. Plant Physiol 117: 63- 72.

Chen, C-M. 1987. Characterization of Cytokinins and Related Compounds by HPLC. Di dalam: Linskens, H.F. and J.F. Jackson (ed.). High Performance Liquid Chromatography in Plant Sciences. Springer-Verlag. Berlin.

Chen, J-H., Czajka, D.R., Lion, L.W., Shuler, M.L. & Ghiorse, W.C. 1995. Trace metal mobilization in soil by bacterial polymers. Environ. Health Perspect 103: 53-58.

Chen, W., Krage, N., Wu, L., Pan, G., Khosrivafard, M. & Chang, A.C. 2008. Arsenic, cadmium, and lead in california cropland soils: role of phosphate and micronutrient fertilizers. J. Environ Qual 37: 689-95.

Chien, S.H., Carmona, G., Prochnow, L.L. & Austin, E.R. 2003. Cadmium avalability from granulated and bulk-blended phosphate-potassium fertilizers. J. Environ. Qual 32: 1911- 1914.

Hindersah, R, Arief, D.H., Sumarni, Y. & Totowarsa. 2003. Produksi hormon sitokinin oleh Azotobacter. Prosiding Kongres dan Seminar Nasional HITI. Padang Juli 2003. 549-555.

Hindersah, R. 2008. Transportasi Kadmium Dari Tanah Ke Pupus Tanaman Selada (Lactuca Sativa) Oleh Eksopolisakarida Azotobacter Sp. Disertasi. Universitas Padjadjaran. Bandung.

Holt, J.G., Krieg, N.R., Shaley, J.T. & William, S.T. 1994. Bergey’s Manual of Determinative Bacteriology. William and Wilkins. Baltimore.

Kurniawati, F., Hindersah, R. & Joy, B. 2006. Pengaruh aplikasi kadmium (Cd), inokulan dan eksopolisakarida Azotobacter sp UT1 terhadap Cd tanah, dan akumulasi Cd pada tajuk bibit selada (Lactuca sativa) . J. Pertanian Tropika. 9: 52-59.

Van Reeuwijk, L.P. 1992. Procedure for Soil Analysis. Fourth Edition. ISRIC. Wageningen. The Netherland.

Vermani, M.V., Kelkarand, S.M. & Kamat, M.Y. 1997. Studies in polysaccharide production and growth of Azotobacter vinelandii MTCC 2459, a plant rhizosphere isolate. Lett. Appl. Microbiol 24: 379383.




DOI: http://dx.doi.org/10.31258/jni.14.01.%25p

Refbacks

  • There are currently no refbacks.


Copyright (c) 2012 Reginawanti Hindersah, Rija Sudirja

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.