Suhu dan Waktu Inkubasi untuk Optimasi Kandungan Eksopolisakarida dan Fitohormon Inokulan Cair Azotobacter sp. LKM6

Reginawanti Hindersah, Rija Sudirja

Abstract


Azotobacter inoculation could play an important role to enhance the effectiveness of bioremediation since bacterialexopolysachharides form a complex with heavy metal. So that metal mobility in soil and its uptake by plantsincreased. Azotobacter also produce phytohormone which induce roots growth and subsequently the uptake ofnutrients. The objective of this research was to obtain optimal incubation temperature and time in Azotobacter sp.LKM6 liquid inoculants production in the fermentor to maximize the synthesis of exopolysachharides andphytohormones. The experiment arranged in Completely Randomized Design consisted of two incubationtemperature (room temperature and 300C). At 24, 36, and 48 hours incubation, the concentration of EPS andphytohormone cytokinin and giberrelin were occurred. The experimental results were 1) the best temperature andincubation time to produce Azotobacter sp. LKM6 liquid for bioremediation of heavy metal-contaminated soil was30oC and 48 hours, and 2) inoculants production at 300C for 48 jam produce liquid inoculants containing 2.87mg L-1 exopolysachharides, 81.0 mg L-1 cytokinins and 18.7 mg L-1 giberrelin, and 13.12 x 108 cell ml-1.

Keywords


azotobacter, bioremediation, cadmium, exopolysachharides, phytohormones

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References


Alloway, B.J. 1995. Cadmium. Di dalam: Alloway, B.J (ed). Heavy Metals in Soils . Glasgow: Blackie Academic and Professional.

Chen, C.M. 1987. Characterization of cytokinins and related compounds by HPLC. Di dalam: Linskens, H.F. and Jackson, J.F. (eds.). High performance liquid Chromatography in Plant Sciences. Berlin: Springer-Verlag.

Chen, J.H., Czajka, D. R,. Lion, L.W., Shuler, M. L. & Ghiorse, W. C. 1995. Trace metal mobilization in soil by bacterial polymers. Environ. Health Perspect 103: 53-58.

Chien, S.H., Carmona, G., Prochnow, L.L. & Austin, E.R. 2003. Cadmium avalability from granulated and bulk-blended phosphate-potassium fertilizers. J. Environ. Qual 32: 1911-1914.

Czajka, D.R., Lion, L.W., Shuler, M. L. & Ghiorse, W. C. 1997. Evaluation of the utility of bacterial extracellular polymers for treatment of metal-contaminated soils. Polymer persistence, mobility, and the influence of lead. Water Res 31: 2827-2839.

Emtiazi, G., Ethemadifar, Z. & Habibi, M.H. 2004. Production of extracellular polymer in Azotobacter and biosorption of metal by exopolymer. Afr. J. Biotech 3: 330-333.

Hindersah, R., Arief, D.H. & Sumarni, Y. 2000. Kontribusi hormonal Azotobacter chroococcum pada pertumbuhan kecambah jagung dalam kultur cair. Prosiding Seminar Nasional Bioteknologi Pertanian, Yogyakarta 6-7 November 2000.

Hindersah, R., Arief, D.H., Sumarni, Y. & Totowarsa. 2003. Produksi hormon sitokinin oleh Azotobacter. Prosiding Kongres dan Seminar Nasional Himpunan Ilmu Tanah Indonesia. Padang, 5-7 Juli 2003.

Hindersah, R., Arief, D. H., Soemitro, S. & Gunarto, L. 2006. Exopolysaccharide extraction from rhizobacteria Azotobacter sp. Proc. International Seminar IMTGT. Medan, 22-23 Juni 2006.

Hindersah, R., Sudirja, R. & Setiawati, M.R. 2007. Akumulasi kadmium pada tajuk tanaman selada dan pakcoy setelah inokulasi Azotobacter sp. Ckl-5. Makalah dipresentasikan pada Seminar Kebudayaan Indonesia Malaysia. Kuala Lumpur 29-31 Mei 2007.

Hindersah, R., Arief, D. H., Soemitro, S. & Gunarto, L. 2008. Peningkatan konsentrasi kadmium pupus selada setelah inokulasi Azotobacter dan aplikasi CdCl2 di Andisols steril dan tidak steril. Prosiding Seminar Nasional dan Dialog sumberdaya Lahan Pertanian, Bogor 18-20 November 2008.

Hindersah, R., Arief, D. H., Soemitro, S. & Gunarto, L. 2008a. Pengaruh CdCl2 terhadap produksi eksopolisakarida Azotobacter. Jurnal Natur Indonesia 12(1): 34-37.

Hindersah, R. 2008. Transportasi kadmium dari tanah ke pupus selada oleh eksopolisakarida Azotobacter. Disertasi Pascasarjana, Bandung: Universitas Padjadjaran.

Holt, J.G., Krieg, N.R., Shaley, J.T. & William, S.T. 1994. Bergey’s Manual of Determinative Bacteriology. Baltimore: William and Wilkins.

Isminarni, F., Wedhastri, S., Widada, J. & Purwanto, B.H. 2007. Penambatan nitrogen dan penghasilan indol asam asetat oleh isolat-isolat Azotohacter pada pH rendah dan alumunium tinggi. Jurnal Ilmu Tanah dan Lingkungan 7: 23-30.

Likhosherstov, L.M., Senchenkova, S.N., Shashkov, A.S., Derevitskaya, V.A., Danilova, I.V. & Botvinko, I.V. 1991. Structure of the major exopolysachharide produced by Azotobacter beijerinckii B-1615. Carbohydr.Res. 222: 233-238.

Lupi, F. M., Fernandes, H.M.L., Tome, M.M., Sa-Correia, I. & Novais, J.M. 1994. Influence of nitrogen source and photoperiod on exopolysaccharide synthesis by the microalga Botryococcus braunii UC 58. Enzyme and Microbial Technology 16: 546-550.

Martyniuk, S. & Martyniuk, M. 2003. Occurance of Azotobacter spp. in some Polish soils. Polish J. Environ.Study 12: 371-374.

Moreno, J., Vargas-Garcýa, C., Lopez, M.J. & Sanchez-Serrano, G. 1999. Growth and exopolysaccharide production by Azotobacter vinelandii in media containing phenolic acids. Journal of Applied Microbiology 86: 439– 445.

Omar, M.N.A., Hanaa, A., Thabet, S.M. & El-Beltagy, E. 2005. Exopolysachharides produced by rhizobia isolated from the rhizosphere of different plants. http://www.arc.sci.eg/ InzstsLabs (15 Oktober 2005).

Petry, S., Furlan, S., Crepeau, M.J., Cerning, J. & Desmazeaud, M. 2000. Factors affecting exocellular polysaccharide production by Lactobacillus delbrueckii subsp. bulgaricus grown in a chemically defined medium. Appl. Environ. Microbiol 66: 3427-3431.

Suhardiyani, M. 2007. Pengaruh sumber dan konsentrasi nitrogen terhadap konsentrasi eksopolisakarida dan jumlah sel inokulan Azotobacter sp. Skripsi Fakultas Pertanian. Bandung: Universitas Padjadjaran.

Vargas-Garcia, M.C., Lopez, M.J., Elorrieta, M.A., Suarez, F Moreno, J. 2003. Properties of polysaccharides produced by Azotobacter vinelandii cultured on 4-hydroxybenzoic acid. J. Appl. Microbiol 94: 389-395.

Vermani, M.V., Kelkarand, S.M. & Kamat, M.Y. 1997. Studies in polysaccharide production and growth of Azotobacter vinelandii MTCC 2459, a plant rhizosphere isolate. Lett. Appl. Microbiol 24: 379-383.




DOI: http://dx.doi.org/10.31258/jni.13.01.%25p

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