Potensi Insektisida Melur (Brucea javanica L. Merr) dalam Mengendalikan Hama Kubis Crocidolomia pavonana (F.) (Lepidoptera: Crambidae) dan Plutella xylostella (L.) (Lepidoptera: Yponomeutidae)

Eka Candra Lina, Arneti Arneti, Djoko Prijono, Dadang Dadang

Abstract


This study was conducted to evaluate the potency of Brucea javanica (melur) for controlling two species ofcrucifer pests, i.e. Crocidolomia pavonana and Plutella xylostella. Melur fruits, twigs, and leaves were extracteddirectly with methanol or sequentially with hexane, ethyl acetate, and methanol. The most active extract was thenfractionated by preparative layer chromatography using hexane, mixtures of ethyl acetate and methanol, andmethanol as eluents. The most active fraction was formulated as EC (emulsifiable concentrate) and WP (wettablepowder) formulations, and tested for their toxicity and antifeedant effect against C. pavonana and P. xylostellalarvae. The results showed that methanol extract of melur fruits was more active than that of twigs and leaves.Fractionation of methanol extract of melur fruits yielded an active fraction which was eluted with ethyl acetate-methanol 9:1. EC and WP formulations of melur fruits were active against C. pavonana larvae with LC50 of 0.39%and 0.21%, respectively. The same formulations were also active against P. xylostella larvae with LC50 of 0.31% and0.54%, respectively. In no-choice tests, the antifeedant effect of the EC formulation on C. pavonana larvae (feedinginhibition [FI]: 70.9%-97.5%) was higher than on P. xylostella larvae (FI: 52.2%-83.9%), but the antifeedant effect ofthe WP formulation on the two species was relatively the same. In a choice test, the EC formulation at LC 85completely inhibited feeding by C. pavonana larvae (FI: 100%).

Keywords


antifeedant, Brucea javanica, crucifer pests, formulation, toxicity

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References


Basana I.R. & Prijono D. 1994. Insecticidal activity of aqueous seed extracts of four species of Annona (Annonaceae) against cabbage head caterpillar, Crocidolomia binotalis Zeller (Lepidoptera: Pyralidae). Bul HPT 7: 50-60.

[CIPAC] Collaborative International Pesticides Analytical Council. 1980. CIPAC Handbook Analysis of Technical and Formulated Pesticides. New York: CIPAC.

Coats J.R. 1994. Risks from natural versus synthetic insecticides. Annu Rev Entomol 39: 489-515.

Govindachari T.R., Kumari G.N.K., Gopalakrishnan G., Suresh G., Wesley S.D. & Sreelatha T. 2000. Insect antifeedant and growth regulating activities of quassinoids from Samadera indica. Fitoterapia 72: 568-571.

Grainge M. & Ahmed S. 1988. Handbook of Plants with Pest Control Properties. New York: J Wiley.

Guo Z., Vangapandu S., Sindelar R.W., Walker L.A. & Sindelar R.D. 2005. Biologically active quassinoids and their chemistry: Potential Leads for drug design. Current Medicinal Chemistry 12: 173-190.

Isman M.B. 2006. Botanical insecticides, deterrents, and repellents in modern agriculture and an increasingly regulated world. Annu Rev Entomol 51: 45-66.

Leskinen V., Polonsky J. & Bhatnagar S. 1984. Antifeedant activity of quassinoids. Journal of Chemical Ecology 10(10).

Lina. E.C, Prijono. J. & Dadang. 2007. Physiological Interferences in the Soybean Armyworms Spodoptera litura F. Caused by Active Fractions of Aglaia harmsiana Extract. (Jurnal Tumbuhan Tropika 6: 1-8.

Lina E.C. 2004. Gangguan fisiologi pada larva Spodoptera litura (F.) (Lepidoptera: Noctuidae) akibat perlakuan dengan fraksi aktif Aglaia harmsiana Perkins (Meliaceae) [tesis]. Bogor: Fakultas Pertanian, Institut Pertanian Bogor.

Metcalf R.L. 1986. The ecology of insecticides and the chemical control of in­sects. In: Kogan M, editor. Ecological Theory and Integrated Pest Man­agement Prac­tice. New York: J Wiley. hlm 251-297.

Mancebo F., Hilje L., Mora G.A. & Salazar R. 2000. Antifeedant activity of Quassia amara (Simaroubaceae) extracts on Hypsipyla grandella (Lepidoptera: Pyralidae) larva. Crop Protection 19: 301-305.

Perry A.S., Yamamoto I., Ishaaya I. & Perry R.Y. 1998. Insecticides in Agriculture and Environment: Retrospects and Prospects. Berlin: Springer.

Polonsky J., Bhatnagar S.C., Griffiths D.C., Picket J.A. & Woodcock C.M. 1989. Activity of quassinoids as antifeedant against aphids. Journal of Chemical Ecology 15. Prakash A. & Rao J. 1997. Botanical Pesticides in Agriculture. Boca Raton: Lewis Publishers.

Prijono D. 1989. Penuntun Praktikum Pestisida dan Teknik Aplikasi. Bogor: Jurusan Hama dan Penyakit Tumbuhan, Fakultas Pertanian, Institut Pertanian Bogor.

Prijono D. 1999. Prospek dan strategi pemanfaatan insektisida alami dalam PHT. Di dalam: Nugroho BW, Dadang, Prijono D, editor. Bahan Pelatihan Pengembangan dan Pemanfaatan Insektisida Alami; Bogor, 9-13 Agustus 1999. Bogor: Pusat Kajian pengendalian Hama terpadu Institut Pertanian Bogor. hlm 1-7.

Sastrosiswojo S. 1995. Sistem pengendalian hama terpadu dalam menunjang agribisnis sayuran. Prosiding Seminar Ilmiah Nasional Komoditas Sayuran; Lembang Bandung, 1995.

Sastrosiswojo S. & Setiawati W. 1993. Hama-hama kubis dan pengendaliannya. di dalam: Permadi AH, Sastrosiswojo S, editor. Kubis. Lembang (Bandung): Balitsa. hlm 39-50.

Schmutterer H, editor. 1995. The Neem Tree, Azadirachta indica A. Juss, and Other Meliaceous Plants: Sources of Unique Natural Products for Integrated Pest Management, Medicine, Industry and Other Purposes. Weinheim: VCH.

Schoonhoven, L.M, Van Loon, J.J.A. & Dicke M. 2005. Insect Plant-Biology. 2 ed, Oxford University Press, New York.

Steel R.G.D., Torrie J.H. & Dickey D.A. 1997. Principles and Procedures of Statistics: A Bio­metri­cal Approach. 3rd ed. Boston: McGraw-Hill.

Zarkani A. 2008. Aktivitas insektisida ekstrak Piper retrofractum Vahl. an Tephrosia vogelii Hook erhadap Crocidolomia pavonana (F.) dan Plutella xylostella (L.) serta keamanan ekstrak tersebut terhadap diadegma semiclausum (Hellen) [tesis]. Bogor: Fakultas Pertanian, Institut Pertanian Bogor.




DOI: http://dx.doi.org/10.31258/jni.12.02.%25p

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