Aktivitas Peroksidase Mutan Pisang Kepok dengan Ethyl Methane Sulphonate (EMS) secara In Vitro 1
Abstract
The activity of peroxidase were observed in leave of banana clone Kepok resulting by treatments EMS. The phenotype of peroxidase wasanalyzed from the banana clones resulting the induced by EMS and without induction after inoculated with pathogen Banana Blood deasesBacterium (BBD). The objectives of research are know variation of activity and band pattern of peroxidase. Induced mutation treatmentsconsist of control, 0.2% EMS for 1 and 3 hours, 0.5% for 1 and 3 hours, each treatments was provided five banana clones. The result showedthat variant value and coefisien variant of peroxidase activity in leaf tissue of clone treated by EMS increased compare to the control. Avariant control is 0.28 with coefisien variant is 29.92%, while variant value of treatment is 8.45 with coefisien variant is 75.75%.Appearance of peroxidase bands on clone resulting by EMS treatments were emerged four band pattern. The first and control band patternhas relative migration distance is 20 and 30, the second 15, 40 and 60, the third is 15, 35 and 50. There are four bands with different relativemigration distance that indicated polymorphic.
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Agrios, G.N. 2005. Plant Pathology. Ed ke-5. San Diego: Academic Press.
Andini, L.S, Sri, H.S., Harsojo & Anastasia, S.D. 1993. Produksi enzim amilase oleh Aspergillus oryzae iradiasi dalam substrat sagu iradiasi. Dalam: Aplikasi Isotop dan Radiasi dalam Bidang Industri, Pertanian dan Lingkungan. BATAN Jakarta .25-30.
Arisoesilaningsih, E. 1984. Resitensi dua varietas Kacang Hijau (Phaseolus radiatus L) terhadap serangan rebah kecambah oleh Rhizoctonia solani. Thesis Sarjana Biologi. Institut Teknologi Bandung.
Bateman, D.F. 1967. Increase in Peroxidase Desearsed Plant Tissue. In Source Book of Laboratory Exercises In Plant Pathology. W. H. Freeman and Co. San Fransisco.
Carusso, C., Chilosi, G., Leonardi, L., Bertini, L., Magro, P., Buonocore, V. & Caporale, C. 2001. A basic peroxidase from wheat kernel with antifungal activity. Phytochemistry 58: 743–750.
Fegan, M. & Prior. 2005. How Complex is the “Ralstonia Solanacearum Species Complex”. Di dalam: Allen, C., Prior, P., Hayward, A.C. (Eds). Bacterial Wilt Disease and The Ralstonia solanacearum Species Complex. St. Paul. USA: APS Press.
Gaspar, T.h. 1984. Integrated Relationship of Biochemical and Physiological Peraoxidase Activities. Di dalam: H. C. Penel and Th. Gaspar (Eds). Molecular and physiologycal asspects of plant peroxidase greppin. University of Geneva Switzerland.
Hardanti, S. & Sukmadjaja, D. 2002. Keragaman beberapa pola pita aksesi Nenas berdasarkan analisis Isozim. J. Bioteknologi Pertanian (7): 2 62-70.
Hadi, H. 2003. Analisis Genetik Sifat Ketahanan Tanaman Karet Terhadap Penyakit Gugur Daun Corynespora. Disertasi Sekolah Pasca Sarjana. Institut Pertanian Bogor.
Imelda, M., Estiati, A. & Hartati, N.S. 2001. Induction of Mutation through Gamma Irradition in three Cultivars of Banana. J. Annalaes Bogorienses 7(2): 75-82.
Jamaluddin, S.A. 1995. Mutation breeding of banana in Malaysia, Musarama 7(1): 5.
Kanazawa, K., Eguchi, N., Iwara, S. & Oetomo. 1981. Electrophoretic Study On Esterase Dan Peroxidase In Stain Blackcrossed With Pollen Of Chinese Cabagge With Reference To Nucleus Substation. Di dalam: Takekar, N. and Griggs, T.D. (Eds). Chinese Cabagge. Proceedings of The First International Cabagge Symposiuim. Avrds. Taiwan 377–383.
Larkin, P.J. 1987. Somaclonal varition history. Methode and meaning. Isma State J. of res 61.
Lassner, M.W. & Orton, T.J. 1983. Detection of Somatic Variation. Di dalam: Tanskley, S.D and Orton, T.J. (Eds). Isozym in plant genetics breeding. Part A. Elsevier Publishing Company Inc. Amsterdam, the Netherlands.
Michael, W., Lassner, Thomas, J. & Orton, T.J. 1983. Detection of Somatic Variation. In: isozym in plant genetics breeding. Part A. Tanskley, S.D and Orton, T.J. (ed). elsevier publishing company inc. Amsterdam: the netherlands.
Micke, A. 1996. 70 years induced mutation to be reconsidered mutation breeding. Newletter 42: 22-24.
Mittal, R.K., Singh, M. & Maaherchanbdani, N. 1997. Isozym diversity index of vigna parent in relation to heterosis for seed yield in green gran (Vigna radiati) Wilczek. Euphytica 36: 61-68.
Novarianto, H. 1994. Pola pewarisan Isozim pada Tanaman Kelapa. J. Penelitian Kelapa 7(1): 30-35.
Potdukhe, N.R. 2004. Effect of Physical and chemical mutagens in M1 generation in red gram (Cajanus cajan) Nat. J. Pl. Improve 6(2): 108-111.
Rahayuningsih, S.T., Djojodirdjo, S., Hartiko, H. & Woejono, M.D. 1989. Kajian peroksidase dan hubungan dengan sifat ketahanan tanaman lada terhadap infeksi Phytoptora palmivora. Jurnal Berkala Penelitian Pascasarjana UGM 467-471.
Ritawati, L. 2001. Polimorfisme Isoenzim beberapa tetua dan Hasil persilangan Karet serta hubungan ketahanannya terhadap Corynespora cassiicola. Thesis. Pasca Sarjana UNAND. Padang.
Saravanan, T., Bhaskaran, R. & Muthusamy, M. 2004. Pseudomonas fluorescens Induced Enzymological Changes in Banana Roots (Cv. Rasthali) against Fusarium Wilt Disease. J. Plant Pathology 3(2): 72-80.
Silverio, G.L., Jhon, T.A., David, D.P., Elvira, G. & David, J.B. 2007. Soluble peroxidase activity in maize endosperm associated with Maize Weevil Resistance. J. Crop Science Society of America 15-22.
Sussiyanti. 2000. Sifat pertumbuhan dan pola pita isozim planlet kultivar tanaman lada (Piper ningrum) pada beberapa tingkat keracunan Al secara in vitro. Thesis Pascasarjana. UNAND. Padang.
Wisnubroto, Syaifulla & Satsijati. 1994. Hasil Penelitian Holtikultura Pelita V. Jakarta: Puslitbangtan.
Yanti, Y., Habazar, T., Mardinus & Mansyurdin. 2008. Perubahan bentuk planlet Pisang Raja Sereh hasil mutasi dengan etie metana sulfmat (EMS) secara in Vitro. J. Natur Indonesia 12(2): 104-108.
Yuhermita. 2002. Fenotip peroksidase pada cabai keriting (Capsicum annumm) dan cabai rawit (Capsicum frutescens). Skripsi Sarjana Biologi FMIPA UNAND. Padang.
DOI: http://dx.doi.org/10.31258/jni.14.01.%25p
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