Pengaruh Ukuran Embriozigot terhadap Regenerasi Beberapa Klon Kakao

Sholeh Avivi, Didik Pudji Restanto, Tri Widyastuti

Abstract


This sesearch identified the responses of size of zygotic embryo of cocoa and to identify the responses of cocoaclone types toward ability to produce somatic embryo. The research was designed by Completely RandomizedFactorial Design within five replications. The first factors were the sizes of used explants, namely small (2-5 mm),average (6-9 mm) and big (10-15 mm). The second factors were cocoa clones, namely RCC 72, Sca 6, KW 162, KW163 and KW 165. The result showed that the best explant was the small size (2-5 mm) of cocoa zygotic embryo. Ithad significantly on number of rooting embryos and number of embryos with buds on rooting media. Clone RCC 72was the most responsive clone and could generate to be plantlet. Clone KW 165 performed the lowest response.


Keywords


cocoa, regeneration, size of embryos

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References


Alemanno, L., Garzon, I., Oliver, G., Dedieu, F., Paulin, D., D’Hauthuile, A., Wapo, L. & Amores, F. 2007. Plant production by somatic embryogenesis from genotypes selected for agronomic traits in Ivory Coast and Ecuador [Poster]. Prosiding 14th International Cocoa Research Conference, 13-18 October 2003, Accra, Ghana-Montpellier: Ci rad, 2003. htt p: //publi cat ions . c irad. fr/une _notice.php?dk=515850 (17 April 2009).

Alemano, L., Ramos, T., Gargadenec, A., Andary, C. & Ferriere, N. 2003. Localization and identification of phenolic compounds in Theobroma cacao L. somatic embryogenesis. Annals of Botany 92: 613-623.

Anonim. 2008. Revitalisasi kakao didukung bibit embriogenesis somatik. http://www.sinartani. com/iptek/revitalisasi-kakao- didukung-bibit-embriogenesis-somatik (13 Nopember 2008). Bindu, M.R. & Mallika, V.K. 2008. Micrografting in cocoa (Theobroma cacao L.). Internat. J. agric. Sci 4(2): 601-603.

Funek, D., Kasran, R., Lock, T.C., Johnsiul, L., Mohammed, A., Hartney, V., Yusof, M.M., Lamin, K. & Tong, L.M. 2007. Biotechnology Research by the Malaysian Cocoa Board: Propagation by somatic embryogenesis. http:// www.koko.gov. m y/CocoaBioTech/INGW orkshop(143- 148).html (7 April 2007).

Issali, A.E., Traoré, A., Ngoran, J.A.K., Koffi, E.K. & Sangaré, A. 2009. Relati ons hi p bet ween s om e phenol ogic al parameters and somatic embryogenesis in Theobroma cacao L. J. Crop Sci. Biotech 11(1): 23-30.

Issali, A.E., Abdoulaye Traoré, Jean Louis Konan, Joseph Mpika, Emile Minyaka, Jeanne Andi Kohi Ngoran. & Abdourahamane Sangaré. 2010. Relationship between five climatic parameters and somatic embryogenesis from sporophytic floral explants of Theobroma cacao L. African Journal of Biotechnology 9(40): 6614-6625.

Li, Z., Traore, A., Maximova, S. & Guiltinan, M.J. 1998. Somatic embryogenesis and plant regeneration from floral explants of cacao (T heobroma cacao L.) us ing Thi di azuron. Department of Horticulture. College of Agricultural Sciences. The Pennsylvania State University Park. Pennsylvania. 16802-4200.

Lopez-Baez, O., Helena, B., Albertus, E. & Vincent, P. 1993. Embriogenesis somatique de cacaoyer Theobroma Cacao la portir de pieces florales. C.R. Acad. Sci. Paris, Science de lavie/Life sciences 316: 579-584.

Mark, G.J. & Maximova, S.N. 2000. Recent Advance in The Tissue Culture Of Cocoa From Somatic Embryos to Bentwood Gardens. ACRI Molecular Biology Laboratory. Tyson Building. The Pennsylvania State University. University Park. PA. 16802.

M asseret, B., Vachet, C., Florin, B., Gianforcaro, M., Fillodeau, A., Brulard, E., Bouquet, J.F., Alvarez, M. & Broun, P. 2008. Propagation of Cocoa (Theobroma cacao L.) by Somatic Embryogenesis and Field Performance of the Trees. Nestle R & D Centre, Tours, France.

Mayolo, G.A., Maximova, S.N., Pishak, S. & Guiltinan, M.J. 2003. Moxalactam as a counter selection antibiotic for Agrobacterium mediated transpormation and its possitive effect on Theobroma cacao somatic embryogenesis. Plant Science 164: 607-615

Oktavia, F., Siswanto., Budiani, A. & Sudarsono. 2003. Embriogenesis somatik langsung dan regenerasi plantlet kopi arabika (Coffea arabica) dari berbagai eksplan. Menara Perkebunan, 71(2): 44-55.

Oliveira Santos, M., Romano, E., Yotoko, K.S.C., Tinoco, M . L. P., Dias, B.B.A. & Arag a˜o , F.J.L . 2005.

Characterisation of the cacao somatic embryogenesis receptor-like kinase (SERK) gene expressed during somatic embryogenesis. Plant Sci. 168: 723-729.

Priyono, Matsaleh. & Suhendi, D. 2000. Daya regenerasi dan morfisme pertumbuhan bibit hasil kultur daun ortotrop dan plagiotrop Coffea canephora melalui embriogenesis somatik. Pelita Perkebunan 16 (2): 65-74.

Luisa, F. Rojas, Julián Londoño, Adriana, M. Gallego, An drea, L . Herrera, Carol ina Agu ilera. & L ucía Atehortúa. 2008. Total polyphenols analysis of mature seeds and tissue cultures of some Colombian cocoa varieties. Actu Biol . [online]. 30(89):117-123. http:// www. sci el o.unal .edu.c o/scielo.php?script=sci_arttext&pid=S0304-35842008000 200001&lng=en&nrm=iso (31 Maret 2011).

Tan, C.L. & Furtek, D.B. 2003. Development of an in vitro regeneration system for Theobroma cacao L. from mature tissues. Plant Sci, 164: 407-412.




DOI: http://dx.doi.org/10.31258/jni.13.03.%25p

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