Protein Haemaglutinin Outer Membran Protein (OMP) 35 kDa sebagai Protein Adhesin Proteus mirabilis pada Vesika Urinaria Kelinci

Enny Suswati, Diana Chusna Mufida

Abstract


Proteus mirabilis is opportunistic and nosocomial pathogen that usually found in clinical specimen from patientswith catheter. The pathogenic mechanism of the bacteria are not fully elucidated especially its potential activity ofthe protein as hemaglutinin and adhesion molecule. The aim of this study is to evaluate the role of 35 kDa outermembrane protein from P. mirabilis. After identification, bacterial isolate of OMP fraction 12,5% SDS-PAGE wereused to isolate OMP followed by hemaglutinin test and invitro adhesion test. The study showed that the 35 kDa OMPof P. mirabilis was a hemaglutinin protein that could agglutinate mice erythrocytes, rabbit erythrocytes, and humangroup O erythrocytes. Hemaglutination test were negative on erythrocytes human blood group A,B, and AB. The 35kDa OMP was also adhesion protein showed by its activity to adhere to the rabbit vesica urinaria epithel receptor.The increase dose of 35 kDa OMP will decrease the amount of P. mirabilis bacteria to adhere to rabbit vesicaurinaria epithel (p< 0,05 ).

Keywords


adhesion protein, fimbriae, hemaglutinin protein, Proteus mirabilis

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References


Ehara, M. Ishibashi, M. Ichinose, Y. Iwanaga, M. Schimotori. & S. Naito. 1986. Purification and Partial Characterisationmaf Fimbriae of Vibrio cholera 0-1; Vaccine. 5(3): 283-286.

Hutson, A.M. Atmar, R.L. Graham. & D.Y. Ester, M.K. 2002. Norwalk Virus Infection and Disease is Assosiated with ABO HistoBlood Group Type; Infecton Disease. 185(9): 1335- 1337.

Li Xin, Johnson. & E.D. Mobley, TLH. 1999. Requirement of MrpH for Mannosa-Resistent Proteus Like fimbriae Mediated Hemaglutination by Proteus mirabilis; Infection and Immunity. 67: 2822- 2833.

Martino, P.D Bertin, Y. Girardeu, J.P. Livrelli, V. Jolly. & B. Daefeuille-Michaud, A. 1995. Molecular Characterization and Adhesive Properties of CF29K, an Adhesin of Kleibsiella pneumonia Strain Involved in Nosocomial Infection’, Infection and Immunity. 63: 4336-4344.

Mathoera, B.R Kok, J.D. Verduin, M.C. & Nijman, M.J.R. 2002. Pathological and Therapeutic Significance of Cellular Invasion by Proteus mirabilis in Enterocystoplasty Infection Stone Model’, Infection and Immunity. 70(12): 7022-7032.

Nagayama, K. Oguchi, T. Arita. & M. Honda, T. 1995. Purification and Characterizations of a Cell-Associated Hemaglutinin of Vibrio parahaemoliticus, Infection and Immunity 63: 1987-1992.

Nakazawa T, Ishibashi M, Konishi H, Takemoto T, Shigeeda M. & Kochiyama T.1989. Hemaglutinin Activity of Campylobacter pylori’, Infection and Immunity. 57: 989-991. Noorhamdani. 2005. Protein Fimbria 16kDa Bakteri Acinobacter baumanni dari Urin Penderita Infeksi Saluran Kemih Berperan sebagai Protein Hemaglutinin dan Adhesin’, Jurnal Ilmu Kedokteran Brawijaya. 21(1): 44-52.

Perepelov, Ujazda E., Senchenkova N.S., Shashkov.A. & Kaca.W. 1999. Strukctural and serological studies on the O-antigen of Proteus mirabilis O14, a new polysaccharide containing 2-[(R)-1-carboxyethylamino] ethyl phosphate, European Journal of Biochemistry. 261(2): 347-353.

Robertson MS, Cade J.F, Savaio H.F. & Clancy R.L. 2003. Helicobacter pylori Infection in the Australian Activity :Current Prevalence and Lack of Assosiation with ABO Nlood Group; Intern Med J. 33(4): 163-167.

Salyer, AA. & Whitt, DD. 2002. Bacterial Pathogenesis A Molecular Approach’, ASM Press Washington DC. 115-127. Sanarto ,S. 2002. Protein Adhesin Salmonella typhi sebagai Faktor virulensi Berpotensi Imunogenik Terhadap Produksi S-IgA Protektif; (Disertasi) Surabaya: Program Pasca Sarjana Universitas Airlangga.

Shankar, SG. Rajjith, MS. Rangananthan, S. Sivaramakharisnana, M. Natarajan, V. & Rasool, SK. 2002. Role of Blood Groups in the Infection rate of Dandruff Caused by Pityrosporium ovale; Indian J Dermatol. 47(1): 21-23.

Smeds, A. Hemman, K. Jakava, V. Pelkonen, S. Imberechts, H. & Palva, A. 2001. Chqaracterization of the Adhesin of Eschericiae coli F18 Fimbriae; Infection and Immunity. 69(5): 7941-7945.

Sumarno. 2000. Karakterisasi Molekuler Protein Adhesi Vibrio cholera O1M094V dan Protein Reseptornya pada Sel Epitel Usus Halus Tikua Putih (Wistar). Studi Patogenesis Vibrio cholera O1M094V. (Disertasi). Surabaya: Program Pasca Sarjana Universitas Airlangga.

Winarsih S, Sumarno. & Roekistiningsih. 1997. Kajian Fungsi dan Sifat Immunogenitas Protein Hemaglutinin 32kDa dan 20 kDa pada Helicobacter pylori; Majalah Kedokteran Universitas Brawijaya. 13(2): 135-141.

Wassif, C. Cheek, D. & Belas, R. 1995. Molecular Analysis of a Melalloprotease from Proteus mirabilis; Journal of Bacteriology. 177(20): 5790-5798.




DOI: http://dx.doi.org/10.31258/jni.12.02.%25p

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