Identifikasi Genetik Mutan Talinum paniculatum JACQ. (GAERTN.) Berdasarkan Marka RAPD

Abstract

Javasom Talinum paniculatum Jacq. (Gaertn.) is one of popular vegetable plants with potential medicinal properties.The plant root is often used as a substitute for ginseng (Panax ginseng L.), and the leaves are used as a vegetableand as a substitute for purslane (Portulaca olaraeae L.). An effort to improve genetic quality of the plant was madethrough induced mutation with ethyl methane sulphonate (EMS). A variety of EMS dosages (0, 0.3%, 0.6%, 0.9%,1.2%, 1.5% dan 1.8%) were applied to javasom seeds to induce mutation for 24 hours at room temperature. Selectedfive putative mutant genotypes of the third generation after mutagen treatment (M3) was analyzed using Ramdomamplified polymorphic DNA (RAD) marker. Twenty RAPD primers generated 185 scorable bands with 78.92% ofthem were polymorphic. Size of the bands varied from 200bp to 2.5kbp. Clustering analysis was performed basedon RAPD profiles using the UPGMA method. The range of genetic distance among individual genotypes was fromfrom 0.31 to 0.58, while genetic variance was 0.29 + 0.17. The five genotypes were proof to be solid mutants.