Isolasi dan Karakterisasi Inulinase dari Aspergillus niger Gmn11.1 Galur Lokal

Saryono Saryono

Abstract


Inulin is a naturally potential polysaccharide used to produced fructose and fructooligosaccharide. Inulinaseknown also as ß-fructosidase can hydrolise inulin to fructose or fructooligosaccharide. Inulinase production fromAspergillus niger Gmn11.1 isolated from dahlia tubers is conducted using medium containing 1% inulin and 0,2%yeast extract. The crude enzyme (filtrate culture) is purified by means of ammonium sulphate salt precipitation,followed by Sephadex G25 gel filtration column chromatography and DEAE cellulose anion exchanger columnchromatography. The result indicated that the enzyme had optimum pH and temperature of 4,6 and 450C, respectivelywith incubation time of 15 hours. The Km and Vmaxs values obtained from this experiment are 20 mg/ml and 0,769mg/ml/hours, respectively. Whereas the relative molecular weight of inulinase was monitored by SDS PAGE is 63KDa.

Keywords


inulinase, inulin, Aspergillus niger

Full Text:

PDF

References


Alexander R. R., J. M. Griffiths & M. L. Wilkinson . 1985. Basic Biochemical Methods. Singapore: John Willey & Sons.

Beatrice, L. Pool_Zobel. 2005. Inulin-type fructan and reduction in colon cancer risk: review of experimental and human data. British Journal of Nutrition 93: 573-590

Gupta A.K., M.Kaur, N. Kaur, & R. Singh. 1992. A comparison of properties of Inulinase of Fusarium oxysporum immobilized on various supports. Journal Chem. Tech. Biotechnology 53:293-296.

Gilbert A. 1957. Colorimetrict Analysis Of Sugar. Method in Enzymology. Vol. III. New York: Academic Press, Inc.

Kazutomo, H., Hayashi K., & Kasumi T. 1990. Purification and properties of inulinase from Arthrobacter globoformis S64- 1. Starch 42(1): 28-30

Lowry, O. H., N. J. Rosenbrough, A. L. Farr & J. R. Randall. 1951. Protein Measurement with Folin Phenol Reagent. J. Biol. Chem. 193: 265-275.

M. Belmari. 1994. Purification and properties of an extracellular inuli nas e l ike  -Fruc tos idases f rom B ac il lus stearothermophylus. Letter Applied Microboal 19(6):410- 413

M . m an zo ni & Cavaz zo ni V. 1991. P urifi cati on and caracterization of sxtracellular inulinase from Kluyveromices ci ceris porus . Lebensm-Wiss Tec nol ogy , Engl and 24(3):236-240.

Nakamura T., Y. Ogata, A. Shitasa, A. Nakamura & K. Ohta. 1995. Continuous production of fructose syrups from Inulin by immobilized inulinase from Aspergillus niger Mutan 817. J. of Fermentation and Bioeng. 80(2): 164-169.

Roberfroid M. B. & N. M. Dezenne. 1998. Dietary fructans. Annu. Rev. Nutrition 18: 117-143.

Saryono, Henny Olivia R. M., Elvita Sepriana, Andi Dahliati, & Ch ain ulfiffah A. M . 2008. A m obil is as i i nuli nase Aspergillus clavatus Gmn11.3 galur lokal Indonesia. Jurnal Natur Indonesia 10(1): 31-35

Saryono, Chainulfiffah A. M., Silvera Devi Sy., Monalisa H.S., & Dasli. 1998. Pemanfaatkan umbi dahlia (Dahlia variabilis) untuk produk si sirup frukt osa (HFS) dan fruktooligosakarida”. Seminar Nasional PBBMI XIV. Bandung.

Saryono, Is Sulistyati P. & Delita Zul. 1997. Identifikasi Jamur Pemecah Inulin pada Umbi Dahlia. Pekanbaru: Lembaga Penelitian Univ. Riau,

Vandamme E. J., & D. G. Derycke. 1983, Microbial inulinase: fermentationprocess, properties and applications. Advances in Appl. Micro. 29: 139-176.

Workman W.E. & D.F.Day. 1983, Enzymatichydrolysis of Inulin tofructose by glutaral dehyde fixed yeast cell. Biotech. & Bioeng. XXVIII:905-910

Zeily N, Ukun MS Soedjanaatmadja, & Abubakar Sidik. 1991. Penuntun Praktikum Biokimia Lanjut. PAU Bioteknologi ITB. Bandung: ITB




DOI: http://dx.doi.org/10.31258/jni.11.01.%25p

Refbacks

  • There are currently no refbacks.


Copyright (c) 2012 Saryono

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.